collagen type iv alpha 1 col4a1 invitrogen  (Thermo Fisher)

Journal: Journal of Pharmaceutical Analysis

Article Title: Signatures of proteomics and glycoproteomics revealed liraglutide ameliorates MASLD by regulating specific metabolic homeostasis in mice

doi: 10.1016/j.jpha.2025.101273

Figure Lengend Snippet: Identification and validation of potential signature molecules affected by liraglutide (Lira). (A) Protein-protein interaction (PPI) analysis of differential proteins and glycoproteins in major pathways. Node size reflects degree value, with red representing glycoproteins and blue representing proteins. Color-coded segments show pathway associations. (B) Messenger RNA (mRNA) expression of Acaa2 , Lamc1 , and Col4a2 (for proteins), and Acox1 , Gclc , and Shmt2 (for glycoproteins). (C) Protein expression of laminin subunit gamma-1 (LAMC1), collagen type IV alpha-2 chain (COL4A2), and acetyl-CoA acyltransferase 2 (ACAA2) (for proteins), and glutamate-cysteine ligase catalytic subunit (GCLC) (for glycoproteins). Data is presented as mean ± standard deviation (SD) ( n = 3 per group). P values were calculated using Student's t -test or one-way analysis of variance (ANOVA) with Tukey's comparisons. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. ns: not significant. PPAR: peroxisome proliferators-activated receptor; ECM: extracellular matrix; ND: normal diet; HFD: high-fat diet; HFDL: HFD + Lira; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: The primary antibodies used in our study were as follows: laminin subunit gamma-1 (LAMC1) (1:8000, 67706-1-Ig; Proteintech Group, Inc., Wuhan, China), collagen type IV alpha-2 chain (COL4A2) (1:5000, 55131-1-AP; Proteintech Group, Inc.), acetyl-CoA acyltransferase 2 (ACAA2) (1:5000, 11111-1-AP; Proteintech Group, Inc.), glutamate-cysteine ligase catalytic subunit (GCLC) (1:6000, 12601-1-AP; Proteintech Group, Inc.), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:100000, 60004-1-Ig; Proteintech Group, Inc.), and α-tubulin (1:6000, PTM-5001; PTM BIO, Hangzhou, China).

Techniques: Biomarker Discovery, Expressing, Standard Deviation

Journal: International journal of molecular sciences

Article Title: Microplastics Exacerbate Cadmium-Induced Kidney Injury by Enhancing Oxidative Stress, Autophagy, Apoptosis, and Fibrosis.

doi: 10.3390/ijms232214411

Figure Lengend Snippet: Figure 5. Effect of MPs and Cd on fibrosis in mouse kidneys. (A–C): Representative images of Sirius Red staining and Masson staining of the kidney after MP and/or Cd exposure. a: Glomerulus, b: Renal tubule. (B,C): Quantitative analysis. (D–G): The levels of collagen type IV alpha 1 chain (COL4A1), transforming growth factor beta 1 (TGF-β1), and alpha smooth muscle actin (α-SMA) were detected using western blotting. (D): Representative western blot image; (E–G): Quantitative analysis. Data are presented as means ± SD from three independent experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to the control group; # p < 0.05, ## p < 0.01, #### p < 0.0001 compared to the Cd group.

Article Snippet: Anti-microtubule associated protein 1 light chain 3 beta (LC3B) (#83506), anti-alpha smooth muscle actin (α-SMA) (#19245), anti-transforming growth factor beta 1 (TGF-β1) (#3711), anti-collagen type IV alpha 1 chain (COL4A1) (#50273), and anti-β-actin (#4970) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining, Western Blot, Control